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               sterile purified water with the assistance of Micropipette and blend extensively. filter the solution 

To put down a procedure for microbial limit testing of Raw materials and Finished product, to determine the microbial load and ensure the absence of specified microorganisms.

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Even further dilutions, where required, are well prepared with the exact read more same diluent. include a enough volume of microbial suspension to obtain an inoculum of not much more than one hundred cfu.

Warmth the mixture of solids and water, with swirling, simply to the boiling stage. Usually do not overheat or sterilize. Transfer simultaneously into a drinking water bathtub maintained at about 50

Decide the possible range of microorganisms from desk provided underneath and record The end result in Annexure-one.

, inspecting the tubes at 3 hours and subsequently at suitable intervals as much as 24 hrs. Test good and destructive controls at the same time Together with the unknown specimens. If no coagulation in any diploma is noticed, the specimen satisfies the necessities in the test for absence of Staphylococcus aureus. Oxidase and Pigment Tests (for Pseudomonas aeruginosa)— With all the support of an inoculating loop, streak representative suspect colonies in the agar surface of Cetrimide Agar Medium about the agar surfaces of website Pseudomonas Agar Medium for Detection of Fluorescin and Pseudomonas Agar Medium for Detection of Pyocyanin contained in petri dishes.

materials can be managed from the adoption of equally thematerials can be controlled with the adoption of equally the

Observe the plate right after completion of incubation interval and depend the full range of colonies in cfu/gm.

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